Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation and Enrichment of Human Adipose-derived …
JoVE Journal
Developmental Biology

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Developmental Biology
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
 

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Article DOI: 10.3791/52181-v • 11:00 min • January 12th, 2015
January 12th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

Tags

Isolation Enrichment Human Adipose-derived Stromal Cells Osteogenesis Bone Marrow-derived Mesenchymal Stromal Cells Stem Cell-based Tissue Engineering Donor Site Morbidity Adipose-derived Stromal Cells ASCs Osteogenic Lineage Cells Tissue Engineering Applications Scaffolds Craniofacial Skeletal Defects Stromal Vascular Fraction Heterogeneous Mixture Surface Marker Profile Osteoprogenitor Cells Critical-sized Calvarial Defects
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter