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Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

13,973 Views

10:00 min

January 12, 2015

DOI:

10:00 min
January 12, 2015

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Summary

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To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

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