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Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
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发育生物学
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JoVE 杂志 发育生物学
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

14,010 Views

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10:00 min

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January 12, 2015

DOI:

10.3791/52087-v

DOI:
10.3791/52087-v

•

10:00 min
January 12, 2015

•

3 Views
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1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology,University of Potsdam, 3Institute of Molecular Biology,Medizinische Hochschule Hannover

Summary

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To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

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ZebrafishEmbryonic HeartDissectionTranscriptional AnalysisCardiac TranscriptomeRNA ExtractionQuantitative Real-time PCRFluorescent LabelingFluidic Shear ForceFiltration

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Cite this Article

Lombardo, V. A., Otten, C., Abdelilah-Seyfried, S. Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis. J. Vis. Exp. (95), e52087, doi:10.3791/52087 (2015).

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