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1Department of Genetics,UMR_S 968, Institut de la Vision, 2Department of Visual Information,UMR_S 968, Institut de la Vision, 3Exploratory Team,UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06,UMR_S 968, Institut de la Vision, 5INSERM, U968,Institut de la Vision, 6CNRS, UMR_7210,Institut de la Vision
Chapters
- 00:05Title
- 01:40Preparations for Retina Sectioning
- 02:46Isolating and Flattening the Retina
- 04:17Preparing the Retina for Sectioning and Sectioning
- 06:35Dissociating and Culturing Photoreceptor Cells
- 09:03Characterizing the Cultured Photoreceptors
- 10:21Conclusion
Neurale nethinde af en mus i alderen 8 dage er på toppen af en 4% gelatine blok. Efter isolering af fotoreceptorlaget (200 um) ved vibratome er fotoreceptorerne podes efter mekanisk og enzymatisk dissociation for kulturen. Fotoreceptorlaget kan anvendes til molekylære biokemiske analyser eller transplantation.
