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Neuroscience

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JoVE Journal Neuroscience
Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
 

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.

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Vibratome Sectioning Mouse Retina Photoreceptor Cultures Central Nervous System Organized Architecture Photoreceptors Rods And Cones Retinal Pigmented Epithelium Ganglion Cells Isolation Gelatin Embedding Vibratome Razor Blade Inner Retina Bipolar Cells Outer Retina Gelatin Removal Papain Activation Fetal Calf Serum DNase I Centrifugation Cell Culture
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