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Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
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December 22, 2014
DOI:
1Department of Genetics,UMR_S 968, Institut de la Vision, 2Department of Visual Information,UMR_S 968, Institut de la Vision, 3Exploratory Team,UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06,UMR_S 968, Institut de la Vision, 5INSERM, U968,Institut de la Vision, 6CNRS, UMR_7210,Institut de la Vision
Summary
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Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.
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Clérin, E., Yang, Y., Forster, V., Fontaine, V., Sahel, J., Léveillard, T. Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures. J. Vis. Exp. (94), e51954, doi:10.3791/51954 (2014).
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