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11:22 min
December 22, 2014
DOI:
10.3791/51954-v
对8岁天小鼠神经视网膜是在4%阿胶块的顶部。感光体层(200微米)通过的vibratome分离后,将感光体后的机械和酶解为培养接种。可用于分子,生化分析或移植的感光层。
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Clérin, E., Yang, Y., Forster, V., Fontaine, V., Sahel, J., Léveillard, T. Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures. J. Vis. Exp. (94), e51954, doi:10.3791/51954 (2014).
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