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的vibratome切片小鼠视网膜准备感光文化
JoVE 신문
신경과학
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JoVE 신문 신경과학
Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
DOI:

11:22 min

December 22, 2014

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Chapters

  • 00:05Title
  • 01:40Preparations for Retina Sectioning
  • 02:46Isolating and Flattening the Retina
  • 04:17Preparing the Retina for Sectioning and Sectioning
  • 06:35Dissociating and Culturing Photoreceptor Cells
  • 09:03Characterizing the Cultured Photoreceptors
  • 10:21Conclusion

Summary

자동 번역

对8岁天小鼠神经视网膜是在4%阿胶块的顶部。感光体层(200微米)通过的vibratome分离后,将感光体后的机械和酶解为培养接种。可用于分子,生化分析或移植的感光层。

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