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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
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Chapters
- 00:05Title
- 01:04Wax Preinfiltration and Embedding
- 06:18Imaging Corals Under Two-photon Fluorescence Microscopy
- 07:293D Volume Rendering and Visualization of SBFI and Two-photon Spectral Fluorescence Data
- 08:55Results: Two-photon Fluorescence 3D Images of Coral Polyps
- 09:58Conclusion
An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.
