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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
 

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

Article DOI: 10.3791/51824-v • 10:39 min • September 5th, 2014
September 5th, 2014 •
1, 1, 1,2, 1,2,3
1Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Geology, University of Illinois at Urbana-Champaign, 3Department of Microbiology, University of Illinois at Urbana-Champaign

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Summary
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An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

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Keywords: Multimodal Optical Microscopy Reef Building Corals Montastraea Annularis Montastraea Faveolata Polyp Tissue Morphology 3D Tissue Structure Fluorescence Microscopy Serial Block Face Imaging Two-photon Confocal Laser Scanning Microscopy Chromatophores Zooxanthellae Chlorophyll
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