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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

12,144 Views

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10:39 min

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September 05, 2014

DOI:

10.3791/51824-v

DOI:
10.3791/51824-v

•

10:39 min
September 05, 2014

•

3 Views
, , ,
1Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 2Department of Geology,University of Illinois at Urbana-Champaign, 3Department of Microbiology,University of Illinois at Urbana-Champaign

Summary

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An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

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Multimodal Optical MicroscopyReef Building CoralsMontastraea AnnularisMontastraea FaveolataFluorescence MicroscopySerial Block Face ImagingTwo-photon Confocal Laser Scanning MicroscopyChromatophoresZooxanthellaeChlorophyll

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Sivaguru, M., Fried, G. A., Miller, C. A. H., Fouke, B. W. Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals. J. Vis. Exp. (91), e51824, doi:10.3791/51824 (2014).

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