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Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
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1Unité d'Immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, Department of Microbiology, Infectiology & Immunology, Faculty of Medicine,Université de Montréal, 2Infectious Diseases Service, CHU Sainte-Justine, Faculty of Medicine,Université de Montréal, 3Department of Paediatrics,Université de Montréal
Chapters
- 00:05Title
- 01:27Coating and Blocking the Plates
- 02:46Cell Plating and Stimulation
- 04:40Spot Development and Detection
- 05:54Results: Representative VZV ELISpot Data
- 07:42Conclusion
Novel generations of functional assays such as gamma interferon (IFN-γ) ELISpot, which detect cytokine production at the single cell level and provide both quantitative and qualitative characterization of T cell responses can be used to assess cell-mediated immune responses directed against varicella zoster virus (VZV).
