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Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

DOI:
10.3791/51643-v

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08:04 min

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July 09, 2014

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, , , , ,
1Unité d'Immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, Department of Microbiology, Infectiology & Immunology, Faculty of Medicine,Université de Montréal, 2Infectious Diseases Service, CHU Sainte-Justine, Faculty of Medicine,Université de Montréal, 3Department of Paediatrics,Université de Montréal

Chapters

  • 00:05Title
  • 01:27Coating and Blocking the Plates
  • 02:46Cell Plating and Stimulation
  • 04:40Spot Development and Detection
  • 05:54Results: Representative VZV ELISpot Data
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

Novel generations of functional assays such as gamma interferon (IFN-γ) ELISpot, which detect cytokine production at the single cell level and provide both quantitative and qualitative characterization of T cell responses can be used to assess cell-mediated immune responses directed against varicella zoster virus (VZV).

Tags

Keywords: IFN-γ ELISpot AssayVaricella-zoster VirusCell-mediated ImmunityUmbilical Cord Blood TransplantationVZV ProphylaxisT Cell ReconstitutionVZV-specific T Cell Response

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