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Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
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Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
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July 09, 2014
DOI:
1Unité d'Immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, Department of Microbiology, Infectiology & Immunology, Faculty of Medicine,Université de Montréal, 2Infectious Diseases Service, CHU Sainte-Justine, Faculty of Medicine,Université de Montréal, 3Department of Paediatrics,Université de Montréal
Summary
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Novel generations of functional assays such as gamma interferon (IFN-γ) ELISpot, which detect cytokine production at the single cell level and provide both quantitative and qualitative characterization of T cell responses can be used to assess cell-mediated immune responses directed against varicella zoster virus (VZV).
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Salem Fourati, I., Grenier, A., Jolette, É., Merindol, N., Ovetchkine, P., Soudeyns, H. Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation. J. Vis. Exp. (89), e51643, doi:10.3791/51643 (2014).
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