/
/
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Chapters
- 00:05Title
- 01:34Preparation of 96-well Daughter Plates with Compounds
- 03:03Preparation of Cell Cultures to Determine Compound Toxicity
- 04:18Preparation of Cell Cultures Infected by rMV2/Luc
- 05:37Luciferase Activity Measurement
- 06:36Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity
- 08:52Results: Identification of Anti-viral Hit Compounds
- 11:06Conclusion
In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.
