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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

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11:34 min

May 05, 2014

DOI:

11:34 min
May 05, 2014

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Summary

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In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.

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