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高通量筛选RNA病毒的广谱化学抑制剂
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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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高通量筛选RNA病毒的广谱化学抑制剂

13,491 Views

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11:34 min

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May 05, 2014

DOI:

10.3791/51222-v

DOI:
10.3791/51222-v

•

11:34 min
May 05, 2014

•

6 Views
, , , , , ,
1Unité de Génomique Virale et Vaccination, Virology Department,Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department,Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department,Institut Pasteur

Summary

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在体外测定法来测量病毒的复制已经由重组RNA病毒表达荧光素酶或能生物发光的其它酶的开发大大提高。这里我们详细介绍，结合麻疹和基孔肯雅病毒，从化学文库分离出广谱抗病毒药物，例如重组菌株的高通量筛选的管道。

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Lucas-Hourani, M., Munier-Lehmann, H., Helynck, O., Komarova, A., Desprès, P., Tangy, F., Vidalain, P. High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses. J. Vis. Exp. (87), e51222, doi:10.3791/51222 (2014).

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