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Méthodologie pour la génération efficace de protéines du virus de la vaccine marquées par fluorescence

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

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Méthodologie pour la génération efficace de protéines du virus de la vaccine marquées par fluorescence

7,244 Views

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:

10.3791/51151-v

•

09:27 min

January 17, 2014

•

7 Views

N. Bishara Marzook*¹, Dean J. Procter*¹, Helena Lynn, Yui Yamamoto, Jacquelyn Horsington, Timothy P. Newsome

¹School of Molecular Bioscience,University of Sydney, ²Department of Medicine,Center for Vascular Research, ³Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Tags

Fluorescent Protein Tagging Vaccinia Virus Recombinant Virus Generation Homologous Recombination Transient Dominant Selection Metabolic Selection Live-cell Microscopy Virus-host Interactions

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