Journal
/
免疫与感染
/
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

7,339 Views

•

09:27 min

•

January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Summary

Automatically generated

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Explore More Videos

Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

Read Article

Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

Download .ris file

Copy

Share Video

.

Copy