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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI:

09:27 min

January 17, 2014

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Bölümler

  • 00:05Başlık
  • 01:34Creating the Recombinant Vector
  • 02:48Recombinant Virus Generation
  • 05:26Plaque Assay: NO Selection
  • 05:59Purity of Viral Stocks: PCR Screening of Genomic DNA
  • 07:23Results: Recombinant Vectors and the VACV Genome
  • 08:43Conclusion

Özet

Otomatik Çeviri

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

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