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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

7,339 Views

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
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1School of Molecular Bioscience,University of Sydney, 2Tıp Fakültesi,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

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A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

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Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

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Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

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