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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
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免疫学と感染
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JoVE Journal 免疫学と感染
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2医学部,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

概要

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A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

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Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

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Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

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