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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI:
10.3791/51151-v

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09:27 min

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January 17, 2014

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, , , , ,
1School of Molecular Bioscience,University of Sydney, 2医学部,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

章

  • 00:05標題
  • 01:34Creating the Recombinant Vector
  • 02:48Recombinant Virus Generation
  • 05:26Plaque Assay: NO Selection
  • 05:59Purity of Viral Stocks: PCR Screening of Genomic DNA
  • 07:23Results: Recombinant Vectors and the VACV Genome
  • 08:43Conclusion

概要

自動翻訳

自動翻訳

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

タグ

Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

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