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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
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October 27, 2013
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Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.
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Jacquier, N., Aeby, S., Lienard, J., Greub, G. Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches. J. Vis. Exp. (80), e51055, doi:10.3791/51055 (2013).
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