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Immunology and Infection
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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
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Immunology and Infection
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JoVE Journal Immunology and Infection
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

English

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14,316 Views

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09:04 min

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October 27, 2013

DOI:

10.3791/51055-v

DOI:
10.3791/51055-v

•

09:04 min
October 27, 2013

•

5 Views
, , ,
1Institute of Microbiology,University Hospital Center and University of Lausanne

Summary

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Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

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Intracellular PathogensLegionellaMycobacteriaChlamydia-like OrganismsAmoebaeAmoebal CocultureAmoebal EnrichmentIsolationCharacterizationClinical SamplesEnvironmental SamplesBacterial GrowthPhagocytosisMacrophagesPathogenic

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Jacquier, N., Aeby, S., Lienard, J., Greub, G. Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches. J. Vis. Exp. (80), e51055, doi:10.3791/51055 (2013).

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