Journal
/
Immunology and Infection
/
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

DOI:
10.3791/51055-v

•

09:04 min

•

October 27, 2013

•

, , ,
1Institute of Microbiology,University Hospital Center and University of Lausanne

Chapters

  • 00:05Title
  • 01:32Amoebal Coculture
  • 04:14Bacterial Staining and Immunofluorescence
  • 06:03Amoebal Enrichment
  • 07:10Representative Amoebal Coculture and Enrichment Results
  • 08:13Conclusion

Summary

Automatic Translation

Automatic Translation

Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

Tags

Keywords: Intracellular PathogensLegionellaMycobacteriaChlamydia-like OrganismsAmoebaeAmoebal CocultureAmoebal EnrichmentIsolationCharacterizationClinical SamplesEnvironmental SamplesBacterial GrowthPhagocytosisMacrophagesPathogenic

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51055?article_type=v

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
check_url/51055?article_type=v

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
check_url/51055?article_type=v

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
check_url/51055?article_type=v

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
check_url/51055?article_type=v

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
check_url/51055?article_type=v

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
check_url/51055?article_type=v

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
check_url/51055?article_type=v

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
check_url/51055?article_type=v

Zika Virus Specific Diagnostic Epitope Discovery
check_url/51055?article_type=v

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Read Article