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Immunologie et infection
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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
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Immunologie et infection
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Journal JoVE Immunologie et infection
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

DOI:
10.3791/51055-v

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09:04 min

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October 27, 2013

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, , ,
1Institute of Microbiology,University Hospital Center and University of Lausanne

Chapitres

  • 00:05Titre
  • 01:32Amoebal Coculture
  • 04:14Bacterial Staining and Immunofluorescence
  • 06:03Amoebal Enrichment
  • 07:10Representative Amoebal Coculture and Enrichment Results
  • 08:13Conclusion

Summary

Traduction automatique

Traduction automatique

Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

Tags

Keywords: Intracellular PathogensLegionellaMycobacteriaChlamydia-like OrganismsAmoebaeAmoebal CocultureAmoebal EnrichmentIsolationCharacterizationClinical SamplesEnvironmental SamplesBacterial GrowthPhagocytosisMacrophagesPathogenic

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