/
/
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
23,067 Views
•
10:38 min
•
February 24, 2014
DOI:
1Department of Entomology,Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 3Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences,Volcani Center
Summary
Automatically generated
We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.
Read Article
Cite this Article
Kliot, A., Kontsedalov, S., Lebedev, G., Brumin, M., Cathrin, P. B., Marubayashi, J. M., Skaljac, M., Belausov, E., Czosnek, H., Ghanim, M. Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues. J. Vis. Exp. (84), e51030, doi:10.3791/51030 (2014).
Copy