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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
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February 24, 2014
DOI:
1Department of Entomology,Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 3Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences,Volcani Center
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We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.
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Kliot, A., Kontsedalov, S., Lebedev, G., Brumin, M., Cathrin, P. B., Marubayashi, J. M., Skaljac, M., Belausov, E., Czosnek, H., Ghanim, M. Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues. J. Vis. Exp. (84), e51030, doi:10.3791/51030 (2014).
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