Journal
/
Immunologie und Infektion
/
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

DOI:
10.3791/51030-v

•

10:38 min

•

February 24, 2014

•

, , , , , , , , ,
1Department of Entomology,Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 3Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences,Volcani Center

Kapitel

  • 00:05Titel
  • 01:26Whitefly, Plant, and Virus Preparations
  • 02:10Whole Mount FISH of Whiteflies
  • 03:58Whitefly Dissection Followed by TYLCV FISH
  • 06:40Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
  • 08:29Results: FISH Images
  • 10:16Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.

Tags

Fluorescence In Situ Hybridization (FISH)Virus LocalizationEndosymbiotic BacteriaSpatial LocalizationVisualizationInfection ProcessReporter Gene SystemsImmunohistochemical MethodsRNA/DNA ProbeFixationHybridizationMicroscopic Visualization

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Read Article