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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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October 15, 2013
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Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.
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Velapatiño, B., Zlosnik, J. E. A., Hird, T. J., Speert, D. P. Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species. J. Vis. Exp. (80), e50730, doi:10.3791/50730 (2013).
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