/
/
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
28,792 Views
•
08:31 min
•
October 15, 2013
DOI:
Summary
Automatically generated
Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.
Read Article
Cite this Article
Velapatiño, B., Zlosnik, J. E. A., Hird, T. J., Speert, D. P. Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species. J. Vis. Exp. (80), e50730, doi:10.3791/50730 (2013).
Copy