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Immunologie und Infektion
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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

28,792 Views

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08:31 min

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October 15, 2013

DOI:

10.3791/50730-v

DOI:
10.3791/50730-v

•

08:31 min
October 15, 2013

•

8 Views
, , ,
1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children,University of British Columbia

Summary

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Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.

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Velapatiño, B., Zlosnik, J. E. A., Hird, T. J., Speert, D. P. Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species. J. Vis. Exp. (80), e50730, doi:10.3791/50730 (2013).

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