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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

14,756 Views

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10:57 min

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July 25, 2013

DOI:

10.3791/50446-v

DOI:
10.3791/50446-v

•

10:57 min
July 25, 2013

•

1 Views
, ,
1School of Life Sciences,Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

Summary

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In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

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RNAiDouble Gene KnockdownGustatory PerceptionProboscis Extension Response (PER) AssayHoney BeesApis MelliferaVitellogenin (vg)Ultraspiracle (usp)Gene ExpressionBehavioral MaturationForagingLearning And Memory

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Cite this Article

Wang, Y., Baker, N., Amdam, G. V. RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera). J. Vis. Exp. (77), e50446, doi:10.3791/50446 (2013).

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