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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
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神经科学
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JoVE 杂志 神经科学
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

DOI:
10.3791/50446-v

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10:57 min

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July 25, 2013

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, ,
1School of Life Sciences,Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

Chapters

  • 00:05Title
  • 02:40RNAi-mediated Double Gene Knockdown
  • 05:54Proboscis Extension Response (PER) Assay
  • 07:36Results: Double Kockdown of Vitellogenin (vg) and Ultraspiracle (usp) Genes affects Gustatory Perception
  • 09:51Conclusion

Summary

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In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

Tags

RNAiDouble Gene KnockdownGustatory PerceptionProboscis Extension Response (PER) AssayHoney BeesApis MelliferaVitellogenin (vg)Ultraspiracle (usp)Gene ExpressionBehavioral MaturationForagingLearning And Memory

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