RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (<em>Apis mellifera</em>)

Ying Wang; Nicholas Baker; Gro V. Amdam

doi:10.3791/50446

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神経科学

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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

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神経科学

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JoVE Journal 神経科学

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

14,757 Views

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10:57 min

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July 25, 2013

DOI:

10.3791/50446-v

DOI:

10.3791/50446-v

•

10:57 min

July 25, 2013

•

1 Views

Ying Wang¹, Nicholas Baker¹, Gro V. Amdam^1,2

¹School of Life Sciences,Arizona State University , ²Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

概要

In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

概要

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