In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.
Zhao, Y., Wayne, N. L. Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish. J. Vis. Exp. (74), e50312, doi:10.3791/50312 (2013).