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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
Capitoli
- 00:05Titolo
- 01:27Dissection of Brains from Adult Medaka
- 02:56Mounting the Brain in a Recording Chamber
- 03:37Electrophysiology: Loose Patch Recording of Action Potential Firing
- 05:36Electrophysiology: Whole Cell Recording of Membrane Potential
- 06:26GnRH3 Neuron Activity in Medaka and Zebrafish
- 07:27Conclusion
In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.
