Journal
/
/
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

DOI:

07:45 min

April 30, 2013

,

Capítulos

  • 00:05Título
  • 01:27Dissection of Brains from Adult Medaka
  • 02:56Mounting the Brain in a Recording Chamber
  • 03:37Electrophysiology: Loose Patch Recording of Action Potential Firing
  • 05:36Electrophysiology: Whole Cell Recording of Membrane Potential
  • 06:26GnRH3 Neuron Activity in Medaka and Zebrafish
  • 07:27Conclusion

Summary

Tadução automática

In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.

Vídeos Relacionados

Read Article