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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
Chapters
- 00:05Title
- 01:40Isolation of PP Cells for Flow Cytometry
- 03:03Antibody Labeling of Cells for Flow Cytometry
- 03:48Preparation of PP Cryosections
- 04:49Cryosectioning
- 05:20Antibody Labeling of Cryosections and Confocal Microscopy Analysis
- 06:11Results: Immunological Analyses of Mouse Peyer’s Patches
- 09:23Conclusion
There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.
