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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
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March 17, 2013
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There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.
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De Jesus, M., Ahlawat, S., Mantis, N. J. Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches. J. Vis. Exp. (73), e50167, doi:10.3791/50167 (2013).
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