/
/
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
32,184 Views
•
09:49 min
•
March 17, 2013
DOI:
Summary
Automatically generated
There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.
Read Article
Cite this Article
De Jesus, M., Ahlawat, S., Mantis, N. J. Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches. J. Vis. Exp. (73), e50167, doi:10.3791/50167 (2013).
Copy