Journal
/
면역학 및 감염병학
/
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

13,938 Views

•

07:50 min

•

July 16, 2012

DOI:

10.3791/4203-v

DOI:
10.3791/4203-v

•

07:50 min
July 16, 2012

•

13 Views
, , , , ,
1Institute for Toxicology and Genetics,Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics,Karlsruhe Institute of Technology, Karlsruhe, Germany

Summary

Automatically generated

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

Explore More Videos

Drug DiscoveryAutomated High-content Inflammation AssayZebrafishSmall Molecule ScreensImmune-modulatory CompoundsHigh-throughput ScreeningChemically Induced Inflammation AssayCopper Sulfate TreatmentGranulocyte Recruitment

Read Article

Cite this Article

Wittmann, C., Reischl, M., Shah, A. H., Mikut, R., Liebel, U., Grabher, C. Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish. J. Vis. Exp. (65), e4203, doi:10.3791/4203 (2012).

Download .ris file

Copy

Share Video

.

Copy