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Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

DOI:
10.3791/4203-v

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07:50 min

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July 16, 2012

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, , , , ,
1Institute for Toxicology and Genetics,Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics,Karlsruhe Institute of Technology, Karlsruhe, Germany

Chapters

  • 00:05Title
  • 01:12Animal Handling and Media Preparation
  • 02:15Drug Treatment
  • 03:00Image Acquisition and Processing
  • 04:02Data Processing
  • 06:32Representative Inflammatory Assay Results
  • 07:23Conclusion

Summary

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Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

Tags

Drug DiscoveryAutomated High-content Inflammation AssayZebrafishSmall Molecule ScreensImmune-modulatory CompoundsHigh-throughput ScreeningChemically Induced Inflammation AssayCopper Sulfate TreatmentGranulocyte Recruitment

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