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Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

13,917 Views

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07:50 min

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July 16, 2012

DOI:

10.3791/4203-v

DOI:
10.3791/4203-v

•

07:50 min
July 16, 2012

•

13 Views
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1Institute for Toxicology and Genetics,Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics,Karlsruhe Institute of Technology, Karlsruhe, Germany

Özet

Automatically generated

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

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Wittmann, C., Reischl, M., Shah, A. H., Mikut, R., Liebel, U., Grabher, C. Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish. J. Vis. Exp. (65), e4203, doi:10.3791/4203 (2012).

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