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Agarose Gel Electrophoresis for the Separation of DNA Fragments
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Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose Gel Electrophoresis for the Separation of DNA Fragments

DOI:
10.3791/3923-v

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07:10 min

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April 20, 2012

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, , ,
1Department of Molecular, Cell, and Developmental Biology,University of California Los Angeles

Chapters

  • 00:05Title
  • 01:31Preparation of the Gel
  • 03:21Setting up Gel Apparatus and Separating DNA Fragments
  • 04:55Observing Separated DNA Fragments
  • 05:43Results: Agarose Gel Electrophoresis of PCR Products
  • 06:23Conclusion

Summary

자동 번역

자동 번역

A basic protocol for the separation of DNA fragments using agarose gel electrophoresis is described.

Tags

Agarose Gel ElectrophoresisDNA FragmentsAgaroseGelationMolecular Sieving PropertiesSucrose Density Gradient CentrifugationPre-cast WellsCurrentPhosphate BackboneNegatively ChargedElectric FieldAnodeMass/charge RatioMolecular WeightBiased ReptationDNA MovementMigration Rate

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