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Agarose Gel Electrophoresis for the Separation of DNA Fragments

JoVE Journal
Biology

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JoVE Journal Biology

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose Gel Electrophoresis for the Separation of DNA Fragments

DOI:

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07:10 min

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April 20, 2012

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Pei Yun Lee, John Costumbrado, Chih-Yuan Hsu, Yong Hoon Kim

¹Department of Molecular, Cell, and Developmental Biology,University of California Los Angeles

Chapters

00:05Title
01:31Preparation of the Gel
03:21Setting up Gel Apparatus and Separating DNA Fragments
04:55Observing Separated DNA Fragments
05:43Results: Agarose Gel Electrophoresis of PCR Products
06:23Conclusion

Summary

Automatic Translation

A basic protocol for the separation of DNA fragments using agarose gel electrophoresis is described.

Tags

Agarose Gel Electrophoresis DNA Fragments Agarose Gelation Molecular Sieving Properties Sucrose Density Gradient Centrifugation Pre-cast Wells Current Phosphate Backbone Negatively Charged Electric Field Anode Mass/charge Ratio Molecular Weight Biased Reptation DNA Movement Migration Rate

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