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Agarose Gel Electrophoresis for the Separation of DNA Fragments

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose Gel Electrophoresis for the Separation of DNA Fragments

DOI:

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07:10 min

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April 20, 2012

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Pei Yun Lee, John Costumbrado, Chih-Yuan Hsu, Yong Hoon Kim

¹Department of Molecular, Cell, and Developmental Biology,University of California Los Angeles

Capítulos

00:05Título
01:31Preparation of the Gel
03:21Setting up Gel Apparatus and Separating DNA Fragments
04:55Observing Separated DNA Fragments
05:43Results: Agarose Gel Electrophoresis of PCR Products
06:23Conclusion

Summary

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A basic protocol for the separation of DNA fragments using agarose gel electrophoresis is described.

Tags

Agarose Gel Electrophoresis DNA Fragments Agarose Gelation Molecular Sieving Properties Sucrose Density Gradient Centrifugation Pre-cast Wells Current Phosphate Backbone Negatively Charged Electric Field Anode Mass/charge Ratio Molecular Weight Biased Reptation DNA Movement Migration Rate

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