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Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
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March 01, 2012
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A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.
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Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration
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Chaturvedi, S., Jung, B., Gupta, S., Anvari, B., Rao, A. Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly. J. Vis. Exp. (61), e3645, doi:10.3791/3645 (2012).
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