/
/
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
12,083 Views
•
09:47 min
•
March 01, 2012
DOI:
Summary
Automatically generated
A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.
Explore More Videos
Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration
Read Article
Cite this Article
Chaturvedi, S., Jung, B., Gupta, S., Anvari, B., Rao, A. Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly. J. Vis. Exp. (61), e3645, doi:10.3791/3645 (2012).
Copy