ジャーナル
/
免疫学と感染
/
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

12,083 Views

•

09:47 min

•

March 01, 2012

DOI:

10.3791/3645-v

DOI:
10.3791/3645-v

•

09:47 min
March 01, 2012

•

3 Views
, , , ,
1Department of Plant Pathology and Microbiology,University of California, Riverside , 2生物工学科,University of California, Riverside

概要

Automatically generated

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.

Explore More Videos

Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration

Read Article

Cite this Article

Chaturvedi, S., Jung, B., Gupta, S., Anvari, B., Rao, A. Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly. J. Vis. Exp. (61), e3645, doi:10.3791/3645 (2012).

Download .ris file

Copy

Share Video

.

Copy