JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
15,524 Views
•
11:34 min
•
May 22, 2011
DOI:
Summary
Automatically generated
We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.
Explore More Videos
Lentivirus-mediatedGenetic ManipulationVisualizationOlfactory Sensory NeuronsIn VivoOlfactory CircuitProjectionAxonsSynaptic TargetsOlfactory BulbMolecular MechanismsGene ExpressionVisualizingSingle OSN AxonsTerminal Arbor MorphologyLentiviral Based TechniqueMicroinjectionOlfactory EpitheliumExpression CassettesGenome IntegrationGreen Fluorescent Protein ExpressionReporter DetectionGene Function StudiesDevelopment PeriodOver-expressionShRNA Mediated Knock-downMorphologiesCell Bodies
Read Article
Cite this Article
Sadrian, B., Chen, H., Gong, Q. Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo. J. Vis. Exp. (51), e2951, doi:10.3791/2951 (2011).
Copy