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神经科学
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Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

DOI:
10.3791/2951-v

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11:34 min

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May 22, 2011

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, ,
1Department of Cell Biology and Human Anatomy, School of Medicine,University of California, Davis

Chapters

  • 00:05Title
  • 00:17Introduction
  • 01:24Preparation
  • 03:22Microinjection
  • 06:17Immunohistochemistry and Visualization
  • 09:36Results
  • 10:19Discussion and Conclusion

Summary

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We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.

Tags

Lentivirus-mediatedGenetic ManipulationVisualizationOlfactory Sensory NeuronsIn VivoOlfactory CircuitProjectionAxonsSynaptic TargetsOlfactory BulbMolecular MechanismsGene ExpressionVisualizingSingle OSN AxonsTerminal Arbor MorphologyLentiviral Based TechniqueMicroinjectionOlfactory EpitheliumExpression CassettesGenome IntegrationGreen Fluorescent Protein ExpressionReporter DetectionGene Function StudiesDevelopment PeriodOver-expressionShRNA Mediated Knock-downMorphologiesCell Bodies

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